EFFECTS OF A NOVEL HEMOGLOBIN-BASED OXYGEN CARRIER ON PERCENT OXYGEN-SATURATION AS DETERMINED WITH ARTERIAL BLOOD-GAS ANALYSIS AND PULSE OXIMETRY

Study objective: Hemoglobin-based oxygen carrier 201 (HBOC-201) is a p olymerized hemoglobin of bovine origin being developed for use in hemo rrhage during surgery or trauma. Pulse oximetry is commonly used in cl inical practice to assess percent saturation of hemoglobin (Spo(2)). T he ability to measure Spo, in the presence of HBOC-201 will be importa nt for the use of this compound in patient care. Methods: We carried o ut a randomized, single-blind, placebo-controlled study at the Upjohn Research Clinics in Kalamazoo, Michigan, with normal, healthy adult me n and women as subjects. The members of four groups of adult subjects (N=24) each received 45 g of HBOC-201 (nine each, men and women) or a control solution (Ringer's lactate) (three each, men and women). Each subject underwent phlebotomy (about 15% of estimated blood volume) fol lowed by 3:1 hemodilution with Ringer's lactate and then either HBOC-2 01 or control solution. An indwelling arterial catheter in the radial artery was used for serial arterial blood gas sampling. Arterial blood gas measurements were made with a cooximeter (instrumentation laborat ories). Fingertip pulse oximetry was used (Criticare 504-US; Criticare , Incorporated). Paired pulse oximetry and arterial blood gas sampling were made serially (at approximately hourly intervals) over 24 hours. Results: The mean +/-SEM difference for Spo(2) for arterial blood gas analysis compared with the pulse oximetry reading in the presence of HBOC-201 was 1.1%+/-.075%; in controls it was .1%+/-.064% (P<.0001 for each) over the 24 hours after dosing. This relationship was constant despite increased concentrations of plasma hemoglobin (between 1 and 2 g/dL [10 to 20 g/L]) in the HBOC-201 groups. Conclusion: Accurate det erminations of Spo, can be made with pulse oximetry in subjects given HBOC-201 over the normal range of Spo(2).