ANALYSIS IN GINGIVAL CREVICULAR FLUID OF 2 OLIGOPEPTIDES DERIVED FROMHUMAN HEMOGLOBIN BETA-CHAIN

HPLC on a reversed phase column: amino acid sequencing and mass spectr ometry were used to determine the structure of two human gingival crev icular exudate oligopeptides (Leu-Thr-Pro-Glu-Glu-Lys-Ser-Ala-Val-Thr- Ala-Leu and Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe) which were shown to have been derived from the beta-chain of hemoglobin. These sequence s may simply represent two degradation products of the beta-chain. How ever, their preservation in an exudate characterized by active peptido lysis may also prompt the question about their possible more specific role.