AN IMMUNOHISTOCHEMICAL STUDY OF COLLAGEN TYPE-III, TYPE-VI AND TYPE-IX IN RABBIT CRANIOMANDIBULAR JOINT TISSUES FOLLOWING SURGICAL INDUCTION OF ANTERIOR DISK DISPLACEMENT

Authors
Citation
Am. Ali et Mm. Sharawy, AN IMMUNOHISTOCHEMICAL STUDY OF COLLAGEN TYPE-III, TYPE-VI AND TYPE-IX IN RABBIT CRANIOMANDIBULAR JOINT TISSUES FOLLOWING SURGICAL INDUCTION OF ANTERIOR DISK DISPLACEMENT, Journal of oral pathology & medicine, 25(2), 1996, pp. 78-85
Citations number
50
Categorie Soggetti
Dentistry,Oral Surgery & Medicine",Pathology
ISSN journal
09042512
Volume
25
Issue
2
Year of publication
1996
Pages
78 - 85
Database
ISI
SICI code
0904-2512(1996)25:2<78:AISOCT>2.0.ZU;2-#
Abstract
The purpose of this study was to determine the effect of surgical indu ction of anterior disk displacement (ADD) on type-III, VI and IX colla gens of the rabbit craniomandibular joint (CMJ) tissues using an immun ohistochemical technique. The right joint was exposed surgically, all discal attachments were severed except for the posterior discal attach ment (bilaminar zone). The disk was then repositioned anteriorly and s utured to the zygomatic arch, The left joint served as a sham-operated control. Ten additional joints were used as non-operated controls. De eply anesthetized rabbits were perfused with 2% buffered formalin 2 we eks (10 rabbits) or 6 weeks (10 rabbits) following surgery. The articu lar disk, bilaminar zone, mandibular condyle and articular eminence we re excised. The last two were decalcified in EDTA. All tissues were th en sectioned at 10 mu m in a cryostat. Sections were incubated with mo noclonal antibodies directed against type-III, VI or IX collagens. Fol lowing incubation in the appropriate FITC-labelled secondary antibodie s, all sections were studied under the fluorescence microscope. The re sults showed a reduction in immunostaining for type-VI and IX collagen s in the condylar cartilage, disk and articular eminence at 2 weeks, f ollowed by an increase in their immunostaining at 6 weeks and the appe arance of a de novo type-III collagen in the condylar cartilage and th e articular eminence. It is concluded that surgical induction of ADD i n the rabbit CMJ leads to alterations in its type-III, VI and IX colla gens.