AN IMMUNOHISTOCHEMICAL STUDY OF COLLAGEN TYPE-III, TYPE-VI AND TYPE-IX IN RABBIT CRANIOMANDIBULAR JOINT TISSUES FOLLOWING SURGICAL INDUCTION OF ANTERIOR DISK DISPLACEMENT
Am. Ali et Mm. Sharawy, AN IMMUNOHISTOCHEMICAL STUDY OF COLLAGEN TYPE-III, TYPE-VI AND TYPE-IX IN RABBIT CRANIOMANDIBULAR JOINT TISSUES FOLLOWING SURGICAL INDUCTION OF ANTERIOR DISK DISPLACEMENT, Journal of oral pathology & medicine, 25(2), 1996, pp. 78-85
The purpose of this study was to determine the effect of surgical indu
ction of anterior disk displacement (ADD) on type-III, VI and IX colla
gens of the rabbit craniomandibular joint (CMJ) tissues using an immun
ohistochemical technique. The right joint was exposed surgically, all
discal attachments were severed except for the posterior discal attach
ment (bilaminar zone). The disk was then repositioned anteriorly and s
utured to the zygomatic arch, The left joint served as a sham-operated
control. Ten additional joints were used as non-operated controls. De
eply anesthetized rabbits were perfused with 2% buffered formalin 2 we
eks (10 rabbits) or 6 weeks (10 rabbits) following surgery. The articu
lar disk, bilaminar zone, mandibular condyle and articular eminence we
re excised. The last two were decalcified in EDTA. All tissues were th
en sectioned at 10 mu m in a cryostat. Sections were incubated with mo
noclonal antibodies directed against type-III, VI or IX collagens. Fol
lowing incubation in the appropriate FITC-labelled secondary antibodie
s, all sections were studied under the fluorescence microscope. The re
sults showed a reduction in immunostaining for type-VI and IX collagen
s in the condylar cartilage, disk and articular eminence at 2 weeks, f
ollowed by an increase in their immunostaining at 6 weeks and the appe
arance of a de novo type-III collagen in the condylar cartilage and th
e articular eminence. It is concluded that surgical induction of ADD i
n the rabbit CMJ leads to alterations in its type-III, VI and IX colla
gens.