MICROCALORIMETRIC STUDY OF MITOCHONDRIA ISOLATED FROM FISH LIVER-TISSUE

Citation
Am. Tan et al., MICROCALORIMETRIC STUDY OF MITOCHONDRIA ISOLATED FROM FISH LIVER-TISSUE, Journal of biochemical and biophysical methods, 31(3-4), 1996, pp. 189-193
Citations number
6
Categorie Soggetti
Biology,Biophysics,"Biochemical Research Methods
ISSN journal
0165022X
Volume
31
Issue
3-4
Year of publication
1996
Pages
189 - 193
Database
ISI
SICI code
0165-022X(1996)31:3-4<189:MSOMIF>2.0.ZU;2-8
Abstract
We determined the thermogenesis curves of mitochondria isolated from f ish liver tissue by using an LKB 2277 Bioactivity Monitor. After isola tion From the fish liver, mitochondria still have activity and can liv e for a long time by using the stored nutrients. We calculated the rec overy rate constants of mitochondria. We found that the thermogenesis curves of mitochondria are similar to those obtained from prokaryotic cells, but not similar to those obtained from eukaryotic cells. We det ermined the metabolic thermogenesis curves of mitochondria isolated fr om two kinds of carp liver tissue, scattered-scaled mirror carp and ha rvest carp. There are some important similarities and some important d ifferences between these thermogenesis curves.