LARGE-SCALE PREPARATION OF PA-OLIGOSACCHARIDES FROM GLYCOPROTEINS USING AN IMPROVED EXTRACTION METHOD

Authors
TOKUGAWA K OGURI S TAKEUCHI M
Citation
K. Tokugawa et al., LARGE-SCALE PREPARATION OF PA-OLIGOSACCHARIDES FROM GLYCOPROTEINS USING AN IMPROVED EXTRACTION METHOD, Glycoconjugate journal, 13(1), 1996, pp. 53-56
Citations number
4
Categorie Soggetti
Biology
Journal title
Glycoconjugate journal → ACNP
ISSN journal
02820080
Volume
13
Issue
1
Year of publication
1996
Pages
53 - 56
Database
ISI
SICI code
0282-0080(1996)13:1<53:LPOPFG>2.0.ZU;2-Q
Abstract
We have developed a new method for the large scale preparation of pyri dylaminated (PA-) oligosaccharides from glycoproteins. Phenol/chlorofo rm extraction was adapted for the removal of protein and excess 2-amin opyridine, improving the efficiency of preparation. From a 2.5 g sampl e of human apo-transferrin, 25-30 mu mol of agalacto biantennary PA-ol igosaccharide could be obtained. By increasing the concentration of PA -oligosaccharide substrate, we were able to detect a very low level of N-acetylglucosaminlytransferase IV activity in CHO cell extracts.