SEQUENCE-ANALYSIS OF THE CDNA FOR THE HUMAN CASEIN KINASE-I-DELTA (CSNK1D) GENE AND ITS CHROMOSOMAL LOCALIZATION

A cDNA clone coding for human casein kinase I (CK1) has been isolated and sequenced. The insert of 1911 bp contained an open reading frame o f 415 amino acids. The entire amino acid sequence of human CK1 was 97% homologous to that of rat CK1 delta, and their sequences in the kinas e domain (284 amino acid residues) were completely identical, predicti ng that the obtained cDNA is for a human homolog of the CK1 delta isof orm (CSNK1D). The considerable similarity in the amino acid sequence o f the kinase domain of human CK1 delta to the Saccharomyces cerevisiae CK1, HRR25 (66%), and to the Saccharomyces pombe CK1, HHP1 (78%), whi ch are involved in the repair of DNA strand break, supports the specul ation that human CK1 delta might also act in DNA metabolism through ex cision and recombinational repair. The human CK1 delta gene was mapped to chromosome 17q25.2-q25.3 by fluorescence in situ hybridization and polymerase chain reaction analysis of the human/rodent hybrid cell pa nels. (C) 1996 Academic Press, Inc.