CLONING AND CHARACTERIZATION OF CPG ISLANDS OF THE HUMAN-CHROMOSOME 1P36 REGION

CpG islands were identified and localized to chromosome 1p36 by means of pulsed-field gel blot hybridization with 1p36-specific microclone p robes. Five CpG islands, designated CpG17, CpG28, CpG60, CpG112a, and CpG112b, were molecularly cloned from corresponding cosmids. All five islands are associated with transcribed sequences, as shown by RNA blo t hybridizations. Screening of cDNA libraries with the island-specific genomic probes led to the isolation of two cDNA clones to date. These encode the human transcription factor E2F-2 and the dominant-negative helix-loop-helix gene ID3, respectively. Pulsed-field gel electrophor esis analysis also revealed that these two genes are located next to e ach other at a distance of about 25 kb. (C) 1996 Academic Press, Inc.