Tj. Kalogeris et al., TRIACYLGLYCEROL AND CHOLESTEROL TRANSPORT DURING ABSORPTION OF GLYCEROL TRIOLEATE VS GLYCEROL TRIELAIDATE, American journal of physiology: Gastrointestinal and liver physiology, 33(2), 1996, pp. 268-276
We used conscious, chronic lymph-fistula rats to compare intestinal ly
mphatic transport of glycerol trioleate (TO) vs. glycerol trielaidate
(TE) and to determine the effect of TO vs. TE on absorption and transp
ort of cholesterol. Rats were implanted with intestinal lymph fistulas
and duodenal cannulas and then given intraduodenal infusions of lipid
emulsions containing purified TO or TE (40 mu mol/h) and cholesterol
(7.8 mu mol/h + 2 mu Ci [C-14]cholesterol). Lymph samples were collect
ed at 0, 2, 4, 5, 6, 7, and 8 h after the start of lipid infusion. Lym
phatic output and luminal and gut wall recovery of radioactive lipid a
t 8 h were quantified. Triacylglycerol(TG) fatty acid isomers did not
affect lymphatic output of TG; lymph TG fatty acid composition and out
put reflected infusate composition. Lymphatic output of cholesterol (m
ass and radioactivity) did not differ between groups; luminal and gut
wall recovery of [C-14] cholesterol was also similar between groups. S
imilar lymphatic transport of TG and cholesterol between triolein- and
trielaidin-infused rats was maintained for up to 16 h after the cessa
tion of an infused lipid load. These results indicate that TO and TE a
re transported into lymph similarly, and that cholesterol absorption a
nd transport are similar irrespective of whether TO or TE is the TG so
urce. The data suggest that trans fatty acid-induced hyper cholesterol
emia is not due to altered intestinal absorption and transport of chol
esterol.