TRIACYLGLYCEROL AND CHOLESTEROL TRANSPORT DURING ABSORPTION OF GLYCEROL TRIOLEATE VS GLYCEROL TRIELAIDATE

Citation
Tj. Kalogeris et al., TRIACYLGLYCEROL AND CHOLESTEROL TRANSPORT DURING ABSORPTION OF GLYCEROL TRIOLEATE VS GLYCEROL TRIELAIDATE, American journal of physiology: Gastrointestinal and liver physiology, 33(2), 1996, pp. 268-276
Citations number
34
Categorie Soggetti
Physiology
ISSN journal
01931857
Volume
33
Issue
2
Year of publication
1996
Pages
268 - 276
Database
ISI
SICI code
0193-1857(1996)33:2<268:TACTDA>2.0.ZU;2-9
Abstract
We used conscious, chronic lymph-fistula rats to compare intestinal ly mphatic transport of glycerol trioleate (TO) vs. glycerol trielaidate (TE) and to determine the effect of TO vs. TE on absorption and transp ort of cholesterol. Rats were implanted with intestinal lymph fistulas and duodenal cannulas and then given intraduodenal infusions of lipid emulsions containing purified TO or TE (40 mu mol/h) and cholesterol (7.8 mu mol/h + 2 mu Ci [C-14]cholesterol). Lymph samples were collect ed at 0, 2, 4, 5, 6, 7, and 8 h after the start of lipid infusion. Lym phatic output and luminal and gut wall recovery of radioactive lipid a t 8 h were quantified. Triacylglycerol(TG) fatty acid isomers did not affect lymphatic output of TG; lymph TG fatty acid composition and out put reflected infusate composition. Lymphatic output of cholesterol (m ass and radioactivity) did not differ between groups; luminal and gut wall recovery of [C-14] cholesterol was also similar between groups. S imilar lymphatic transport of TG and cholesterol between triolein- and trielaidin-infused rats was maintained for up to 16 h after the cessa tion of an infused lipid load. These results indicate that TO and TE a re transported into lymph similarly, and that cholesterol absorption a nd transport are similar irrespective of whether TO or TE is the TG so urce. The data suggest that trans fatty acid-induced hyper cholesterol emia is not due to altered intestinal absorption and transport of chol esterol.