METHOD DEVELOPMENT AND OPTIMIZATION FOR SMALL ION CAPILLARY ELECTROPHORESIS

Method development and optimization procedures in capillary electropho resis for small, mainly inorganic, ions are discussed. The selection o f initial conditions and the optimization of selectivity are reviewed. The variables are grouped in three classes: variables related to the system, the sample, and the buffer electrolyte. The variables related to the buffer electrolyte are discussed in detail, as they are the one s primarily considered in selectivity adjustment. After dealing with t he separation mechanisms for anions and cations, migration modelling p rocedures (both empirical and theoretical) are discussed. Examples of applications are provided.