The structure of the O-specific side chain of the E, coli O26 lipopoly
saccharide has been investigated. Based on sugar and methylation analy
ses, and 2D NMR spectroscopy employing HMBC experiments, it is conclud
ed that the polysaccharide is composed of trisaccharide repeating unit
s having the following structure: --> 3)-alpha-L-Rha p-(1 --> 4)-alpha
-L-Fuc pNAc-(1 --> 3)-beta-D-Glc pNAc-(1 -->