MODIFICATION OF THE ACTION OF PACLITAXEL ON A HUMAN DIFFERENTIATED COLON-CANCER CELL-LINE AFTER LONG-TERM PRETREATMENT AT VERY-LOW DOSE

Citation
G. Carles et al., MODIFICATION OF THE ACTION OF PACLITAXEL ON A HUMAN DIFFERENTIATED COLON-CANCER CELL-LINE AFTER LONG-TERM PRETREATMENT AT VERY-LOW DOSE, Bulletin du cancer, 83(2), 1996, pp. 135-138
Citations number
8
Categorie Soggetti
Oncology
Journal title
ISSN journal
00074551
Volume
83
Issue
2
Year of publication
1996
Pages
135 - 138
Database
ISI
SICI code
0007-4551(1996)83:2<135:MOTAOP>2.0.ZU;2-U
Abstract
Taxoids (paclitaxel, Taxol(R) and docetaxel, Taxotere(R)), drugs which stabilize microtubules, demonstrate marked activity against ovarian, brain and lung cancer We investigated, on the human colon adenocarcino ma HT29-D4 cell line, firstly the effects of taxoids in function of th e differentiation state and secondly the effects of a low dose of pacl itaxel on the differentiation process. The cellular parameters studied were microtubular network, cell cycle and cell tubulin content. Undif ferentiated cells treated with paclitaxel or docetaxel (10-100 nM) cla ssically induced bundles in interphasic cells and pseudoasters in mito tic cells, arrest in cell cycle in G(2)M and increase in tubulin conte nt. Inversely, no modification was observed in differentiated cells af ter similar taxoid treatment. Long-term low-dose pretreatment of diffe rentiated cells restitutes some sensitivity to taxoids since these cel ls become responsive to further taxoid treatment, as reflected by modi fications of the microtubule network.