SIMULTANEOUS DETERMINATION OF FELBAMATE AND 3 METABOLITES IN RAT AND DOG PLASMAS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

An isocratic liquid chromatographic method employing one extraction st ep and a 150 mm x 4.6 mm I.D. Spherisorb ODS2, 3-mu m HPLC column usin g UV-absorbance detection at 210 nm has been developed for the quantit ation of felbamate and three felbamate metabolites in 0.100-ml aliquot s of rat and dog plasmas. The linear quantitation range in rat plasma is 0.195-200 mu/ml for felbamate; 1.563-200 mu g/ml for the p-hydroxy metabolite; 0.391-200 mu g/ml for the 2-hydroxy metabolite; and 0.098- 200 mu g/ml for the monocarbamate metabolite. The linear quantitation range in dog plasma is 0.195-200 mu g/ml for felbamate; 0.781-200 mu g /ml for the p-hydroxy metabolite; 0.195-200 mu g/ml for the 2-hydroxy metabolite; and 0.098-200 mu g/ml for the monocarbamate metabolite.