MICELLAR ELECTROKINETIC CHROMATOGRAPHY OF HYDROXYPROLINE AND OTHER SECONDARY AMINO-ACIDS IN BIOLOGICAL SAMPLES WITH LASER-INDUCED FLUORESCENCE DETECTION

Authors
CHAN KC JANINI GM MUSCHIK GM ISSAQ HJ
Citation
Kc. Chan et al., MICELLAR ELECTROKINETIC CHROMATOGRAPHY OF HYDROXYPROLINE AND OTHER SECONDARY AMINO-ACIDS IN BIOLOGICAL SAMPLES WITH LASER-INDUCED FLUORESCENCE DETECTION, Journal of chromatography. Biomedical applications, 622(2), 1993, pp. 269-273
Citations number
29
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography. Biomedical applications → ACNP
ISSN journal
03784347
Volume
622
Issue
2
Year of publication
1993
Pages
269 - 273
Database
ISI
SICI code
0378-4347(1993)622:2<269:MECOHA>2.0.ZU;2-B
Abstract
Micellar electrokinetic chromatography (MEKC) with laser-induced fluor escence (LIF) was used for the rapid and sensitive detection of hydrox yproline in serum and hydrolyzed urine that were pre-column derivatize d with 9-fluorenylmethyl chloroformate (FMOC). The application of the combined o-phthalaldehyde (OPA)/FMOC derivatization in MEKC for the se lective detection of secondary amino acids in biological samples is in vestigated.