SPONGE FATTY-ACIDS .5. CHARACTERIZATION OF COMPLETE SERIES OF 2-HYDROXY LONG-CHAIN FATTY-ACIDS IN PHOSPHOLIPIDS OF 2 SENEGALESE MARINE SPONGES FROM THE FAMILY SUBERITIDAE - PSEUDOSUBERITES SP AND SUBERITES MASSA

Phospholipid fatty acid composition was studied in two Senegalese spon ges of the family Suberitidae. More than fifty acids were identified i n either Pseudosuberites sp. or Suberites massa. A series of 2-hydroxy long-chain fatty acids (C-22 to C-27) accounted for almost 50% of the total acid mixture of Pseudosuberites sp., including 2-hydroxyhexacos anoic acid (26%) and the unusual 2-hydroxy-heptacosanoic acid not yet reported in any sponge. A series of 3-hydroxy short-chain fatty acids was also detected in this sponge. Two new fatty acids were identified in these sponges, namely 4,8-dimethyldecanoic and 12-methyl-(Z)-6-trid ecenoic, in addition to the rare 13-methyl-(Z)-4-tetradecenoic. Su. ma ssa contained mainly 5,9-dienoic and trienoic demospongic acids. For t he first rime, 2-hydroxy long-chain fatty acid methyl esters were anal yzed by gc-Ft-ir, and the corresponding N-acyl pyrrolidide derivatives by gc-ms.