Lp. Clergeau et al., HEALING RESPONSE TO ANORGANIC BONE IMPLANTATION IN PERIODONTAL INTRABONY DEFECTS IN DOGS .1. BONE REGENERATION - A MICRORADIOGRAPHIC STUDY, Journal of periodontology, 67(2), 1996, pp. 140-149
THE PURPOSE OF THE PRESENT STUDY was to explore the regenerative poten
tial of anorganic bone plus collagen (AB-C) in experimental intrabony
defects. Eight healthy female beagle dogs, 3 to 4 years old and weighi
ng 15 to 16 kilos, were used. After extraction of the mandibular third
premolars (P3), surgical defects were created and inflammation induce
d by placement of cotton and steel braids. Eight weeks later, the brai
ds were removed. The experimental lesions thus obtained were either tr
eated by plain flap curettage (group 1: control) or were, in addition,
implanted with AB-C (group 2: experimental). Blocks of AB-C alone wer
e observed by scanning electron microscopy (SEM). The results show tha
t the surface of the particles have the characteristics of a bone tiss
ue. These particles are gathered together with a fibrillar network. Si
x, 18, and 36 weeks postoperative (PO), non-decalcified specimens from
both groups were examined histologically by contact microradiography.
In group 1? no significant bone regeneration was observed at 6, 18, o
r 36 weeks PO. In group 2, trabeculae undergoing mineralization and ci
rcumscribing dense particles above the reference notch were seen at 6
weeks PO; 18 and 36 week specimens showed significant bone regeneratio
n with more or less dense remaining particles, The periodontal ligamen
t space was always clear and the only signs of ankylosis noticed were
deep in the notch on one 18 week group 2 specimen and on one 36 week g
roup 1 specimen.