DNA-DNA hybridization was used to compare seven taxa from five avian o
rders, with an alligator as outgroup. Complete matrices of Delta T50H
and Delta NPH (both symmetrized and unsymmetrized) gave the same FITCH
topology, which was supported in 100% of bootstrapped and jackknifed
trees. The outgroup alligator rooted the tree between anseriform-galli
form and coliiform-strigiform-columbiform clades, and resolution withi
n the latter favored a strigiform-columbiform association. In contrast
, Delta T-m gave differing and more poorly supported FITCH resolutions
for deeper nodes because the distances were compressed due to greatly
reduced NPHs. An F-ratio test between FITCH and KITSCH trees based on
symmetrized Jukes-Cantor-corrected Delta T(50)Hs indicated significan
t rate variation among the lineages. Despite this result, the UPGMA al
gorithm applied to symmetrized data gave a topology identical to the D
elta T50H and Delta NPH FITCH trees, whether or not the outgroup allig
ator was included. However, phenograms calculated from unsymmetrized D
elta s of all three indices associated Bubo and Colius, as did the FIT
CH tree based on a completed matrix reconstructed from Sibley and Ahlq
uist's original data. Thus, our results support Sibley and Ahlquist's
use of Delta T50H to assess ordinal patterns in avian phylogeny, repli
cate a portion of their ''tapestry'' based on the same DNA-DNA hybridi
zation technique, and show that for these taxa least-squares and phene
tic algorithms generate much the same topology.