Bk. Amoah et al., THE USE OF THE RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE TO IDENTIFYMATING GROUPS IN THE FUSARIUM SECTION LISEOLA, Plant Pathology, 45(1), 1996, pp. 115-125
The random amplified polymorphic DNA (RAPD) technique was used to dete
rmine the mating groups of several members of the Fusarium section Lis
eola recovered from maize, rice and sorghum collected from different l
ocations in Ghana. Three mating groups were identified, A, D and F, of
which all A and F isolates were confirmed by mating studies. Fertile
crosses were also obtained in crosses involving two of the isolates id
entified as belonging to the D population. Variability within the A po
pulation isolated from seeds and stem-bases of maize was investigated
to determine whether the sub-structuring of this population was relate
d to the host tissue from which the isolates were obtained. The relati
ve merits of the RAPD procedure, compared to the mating procedure, for
determining the mating affiliations of isolates and for more detailed
analyses of isolates within a population, as well as its possible adv
antages over established RFLP methodologies are discussed.