Y. Kamano et al., AN IMPROVED SOURCE OF BRYOSTATIN-10, BUGULA-NERITINA FROM THE GULF-OF-AOMORI, JAPAN, Journal of natural products, 58(12), 1995, pp. 1868-1875
Bryostatins have been isolated from diverse Japanese coastal specimens
of Bugula neritina guided by inhibitory activity against fertilized s
ea urchin egg cell division. B. neritina from the Gulf of Aomori, Japa
n, has been found to contain bryostatin 10 [1b] in high yield (10(-3)%
) for this class of compounds. The H-1- and C-13-nmr signals of bryost
atin 10 [1b] were reassigned by 2D nmr techniques. The conformation of
bryostatin 10 [1b] in solution was revealed by nmr studies. This comp
ound also exhibited activity in a steroidogenesis assay by increasing
the production of adrenocortical hormones nearly twofold.