THE RESOLUTION OF DYE BINARY-MIXTURES BY BIVARIATE CALIBRATION USING SPECTROPHOTOMETRIC DATA

A new, simple spectrophotometric method for resolution of dye binary m ixtures is proposed in this work. A simple mathematical algorithm was designed, in which the data are used from four linear regression calib ration equations: two calibrations for each component at two selected wavelengths. The method of Kaiser was applied for the selection of the optimum two-wavelength sets for all mixtures under study. The recover y experiments were carried out in ten mixtures of the following dyes: Tartrazine, Amaranth, Erythrosin B, Sunset Yellow and Allura Red. The obtained results were compared with the results of a commonly used der ivative spectrophotometric procedure (zero-crossing technique). The st atistical evaluation of the method bias was performed and it was concl uded that the proposed methodology may be competitive with the derivat ive procedure for the resolution of such dye binary mixtures.