Integration of information between tyrosine kinase(1) and G-protein-me
diated pathways(2) is necessary, but remains poorly understood. Here w
e use cells from transgenic mice harbouring inducible expression of RN
A antisense to the gene encoding G(i alpha 2) (refs 3, 4) to show that
G(i alpha 2) is critical for insulin action. G(i alpha 2) deficiency
in adipose tissue and liver produces hyperinsulinaemia, impaired gluco
se tolerance and resistance to insulin in vivo. Insulin resistance aff
ects glucose-transporter activity and recruitment, counterregulation o
f lipolysis, and activation of glycogen synthase, all of which are car
dinal responses to insulin(5). G(i alpha 2) deficiency increases prote
in-tyrosine phosphatase activity and attenuates insulin-stimulated tyr
osine phosphorylation of IRS (insulin-receptor substrate 1) in vivo. G
(i alpha 2) deficiency creates a model for the insulin resistance char
acteristic of noninsulin-dependent diabetes mellitus (NIDDM)(6), impli
cating G(i alpha 2) as a positive regulator of insulin action.