Rs. Koduri et al., OXIDATION OF 1,2,4,5-TETRAMETHOXYBENZENE BY LIGNIN PEROXIDASE OF PHANEROCHAETE-CHRYSOSPORIUM, Archives of biochemistry and biophysics, 326(2), 1996, pp. 261-265
We have reinvestigated the lignin peroxidase-catalyzed oxidation of 1,
2,4,5-tetramethoxybenzene (TMB) by using presteady-state and steady-st
ate kinetic methods, Our presteady-state kinetic results show that the
reaction of compound I with TMB obeyed second order kinetics with a r
ate constant of 1.1 X 10(7) M(-1) s(-1) The reaction of compound II wi
th TMB exhibits a hyperbolic concentration dependence with a K-d of 16
mu M and k = 24 s(-1). The stoichiometry of TMB oxidation during stea
dy state is two TMB cation radicals formed per H2O2 consumed, These re
sults clearly show that TMB is a good substrate for both compounds I a
nd II of lignin peroxidase. (C) 1996 Academic Press, Inc.