DETERMINATION OF 1,4-BENZODIAZEPINES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROSPRAY TANDEM MASS-SPECTROMETRY

Authors
KLEINSCHNITZ M HERDERICH M SCHREIER P
Citation
M. Kleinschnitz et al., DETERMINATION OF 1,4-BENZODIAZEPINES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROSPRAY TANDEM MASS-SPECTROMETRY, Journal of chromatography B. Biomedical applications, 676(1), 1996, pp. 61-67
Citations number
20
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
676
Issue
1
Year of publication
1996
Pages
61 - 67
Database
ISI
SICI code
Abstract
A sensitive and selective method to determine diazepam, N-desmethyldia zepam, nitrazepam, flunitrazepam and medazepam in human serum and urin e was established employing solid-phase extraction and high-performanc e liquid chromatography-electrospray tandem mass spectrometry (HPLC-ES I-MS-MS) with the selected reaction monitoring (SRM) mode. Using diaze pam-d(5) and N-desmethyldiazepam-d(5) as internal standards, recoverie s between 90.4 and 109.7% were determined. The routine quantification limit was set at 2 ng/ml for serum and urine at a signal-to-noise rati o of 10:1.