DETECTION AND DETERMINATION OF THE MAJOR METABOLITES OF [H-3] CYTOSINE-ARABINOSIDE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
BRAESS J PFORTNER J KAUFMANN CC RAMSAUER B UNTERHALT M HIDDEMANN W SCHLEYER E
Citation
J. Braess et al., DETECTION AND DETERMINATION OF THE MAJOR METABOLITES OF [H-3] CYTOSINE-ARABINOSIDE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 676(1), 1996, pp. 131-140
Citations number
13
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
676
Issue
1
Year of publication
1996
Pages
131 - 140
Database
ISI
SICI code
Abstract
An ion-pair high-performance liquid chromatographic assay involving so lid-phase scintillation detection was established for the rapid identi fication and determination of all major metabolites of tritium-labelle d cytosine arabinoside (Ara-C) in an in vitro system. In a single run of 50 min, Ara-C, Ara-CMP, Ara-CDP-choline, Ara-CDP, Ara-U, Ara-UMP, A ra-CTP, Ara-UDP and Ara-UTP can be measured. The method is fast, sensi tive, with limits of detection ranging from 40 to 200 pg (absolute), a nd highly reproducible.