EFFECTS OF A BONE-INDUCING AGENT DERIVED FROM A CULTURED HUMAN OSTEOSARCOMA CELL-LINE AFTER ORTHOTOPIC AND HETEROTOPIC IMPLANTATION IN THE DOG

BIA, a Bone Inducing Agent extracted from a cultured osteosarcoma cell line is highly osteoinductive in rodents. BIA was evaluated and compa red with autogenous cancellous bone graft and bovine collagen after or thotopic and heterotopic implantation in Beagles. Each implant was pla ced in the latissimus dorsi muscle of five Beagles and in femoral cort ical defects of four Beagles. Bone formation was evaluated by radiogra phy, dual energy X-ray absorptiometry, histology and histomorphometry. BIA did not induce bone formation after heterotopic implantation and did not promote bone healing after orthotopic implantation in dogs.