M. Decuyper et al., CATALYTIC DURABILITY OF MAGNETOPROTEOLIPOSOMES CAPTURED BY HIGH-GRADIENT MAGNETIC FORCES IN A MINIATURE FIXED-BED REACTOR, Biotechnology and bioengineering, 49(6), 1996, pp. 654-658
Cytochrome c-oxidase, used as a model membrane-bound enzyme, was embed
ded in phospholipid bilayer membranes, attached to nanometer-sized Fe3
O4 colloids (so-called magnetoliposomes). In comparison with the lipid
-depleted free enzyme, both the activity and the enzymatic stability o
f the complexes, stored at 4 degrees C, were considerably enhanced. Th
ese beneficial properties of magnetoproteoliposomes have been successf
ully exploited in a magnetically controlled fixed-bed bioreactor, oper
ating in a continuous flow regime. (C) 1996 John Wiley & Sons, Inc.