BINDING-SITE ANALYSIS OF C-MYB - SCREENING OF POTENTIAL BINDING-SITESBY USING THE MUTATION MATRIX DERIVED FROM SYSTEMATIC BINDING-AFFINITYMEASUREMENTS

The c-Myb oncoprotein is known to bind to multiple sites in the promot ers of target genes. We have developed a protocol to screen the bindin g site of c-Myb by using the systematic binding data derived from meas urements of binding affinity for oligonucleotide containing a known My b-binding site and its complete single mutants. We first applied the m ethod to predict the binding affinity for the known binding sites and compared with available experimental data. The predicted binding sites agree with many putative binding sites of known target promoters. How ever, there are some binding sites not predicted by the analysis. Thes e sequences deviate from the consensus sequence derived from the bindi ng analyses. In the light of the structure of Myb-DNA complex, these r esults indicate that different DNA-binding modes may be used by c-Myb to recognize different classes of binding sites, We also screened the sequence database for potential Myb-binding sites, and found sequences of several promoters that have not been identified experimentally but could be the target for c-Myb.