A MICRODIALYSIS FIBER BASED SAMPLER FOR FLOW-INJECTION ANALYSIS - DETERMINATION OF L-LACTATE IN BIOFLUIDS BY AN ELECTROCHEMICALLY SYNTHESIZED BILAYER-MEMBRANE BASED BIOSENSOR

Authors
PALMISANO F CENTONZE D QUINTO M ZAMBONIN PG
Citation
F. Palmisano et al., A MICRODIALYSIS FIBER BASED SAMPLER FOR FLOW-INJECTION ANALYSIS - DETERMINATION OF L-LACTATE IN BIOFLUIDS BY AN ELECTROCHEMICALLY SYNTHESIZED BILAYER-MEMBRANE BASED BIOSENSOR, Biosensors & bioelectronics, 11(4), 1996, pp. 419-425
Citations number
17
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biophysics
Journal title
Biosensors & bioelectronics → ACNP
ISSN journal
09565663
Volume
11
Issue
4
Year of publication
1996
Pages
419 - 425
Database
ISI
SICI code
0956-5663(1996)11:4<419:AMFBSF>2.0.ZU;2-U
Abstract
A microdialysis fibre based, low volume sampler is described which can be used in flow injection analysis (FIA) when an on-line dilution of the sample and/or removal of high molecular weight interferents is req uired. This device used in combination with a lactate amperometric bio sensor based on lactate oxidase electrochemically immobilised in a bil ayer membrane of poly(o-phenylendiamine) and overoxidized poly(pyrrole ) permits the extension of the linear range of response up to 10 mM la ctate. Combining microdialysis sampling with FIA and amperometric dete ction at an interference-free and fast-response biosensor, lactate det ermination in complex media such as serum, milk and yoghurt can be eas ily achieved with a high sample throughput and no sample pre-treatment .