NUCLEIC-ACID VACCINE ENCODING GD2 PROTECTS MICE FROM HERPES-SIMPLEX VIRUS TYPE-2 DISEASE

Nucleic acid vaccinations with plasmids pWW65, containing the sequence for herpes simplex type 2 (HSV-2) gD2, and pRSVnt, lacking the go seq uence, were studied. Groups of mice were immunized with pWW65 alone, p WW65 plus 1,25-dihydroxyvitamin-D3 (D3), or pRSVnt, Clinical disease ( vaginitis), serum and vaginal washing antibody levels, and vaginal was hing virus titers were measured after intravaginal HSV-2 challenge, No animals (0/10) in the pWW65 + D3 group, 6/10 animals in the pWW65 gro up, and 10/10 animals in the pRSVnt group developed severe disease by postchallenge day 13 (P < .001, P = .04 vs. pRSVnt), Virus titers in v aginal washings were significantly reduced in the pWW65 and pWW65 + D3 groups versus the pRSVnt group (P < .001), Increasing levels of serum anti-gD2 antibodies were measured 2 and 6 days after challenge among animals in the pWW65 and pWW65 + D3 groups but not among animals in th e pRSVnt group, Vaccinations with a plasmid containing the gD2 gene ar e immunogenic and provide some protection from HSV-2-induced disease.