INCREASED HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REPLICATION IN HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS INDUCED BY ETHANOL - POTENTIAL IMMUNOPATHOGENIC MECHANISMS
O. Bagasra et al., INCREASED HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REPLICATION IN HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS INDUCED BY ETHANOL - POTENTIAL IMMUNOPATHOGENIC MECHANISMS, The Journal of infectious diseases, 173(3), 1996, pp. 550-558
Previous studies have shown that alcohol ingestion significantly incre
ases human immunodeficiency virus type 1 (HIV-1) replication in periph
eral blood mononuclear cells (PBMC) isolated and infected with HIV-1 i
n vitro, Whether the increased replication of HTV-1 observed after alc
ohol ingestion was due to unknown factors released from the gastrointe
stinal tract during alcohol ingestion or to certain metabolites produc
ed by intestinal flora that degraded alcohol was investigated, In addi
tion, cellular mechanisms involved in the increased replication of HIV
-1 after alcohol exposure were evaluated, Twelve healthy HIV-l-seroneg
ative subjects abstained from alcoholic beverages for greater than or
equal to 10 days. Nine were infused with 500 mg/kg ethanol (7.5% at 20
mL/kg/h) in saline, whereas 3 were infused with saline alone, Compare
d with saline-infused subjects, ethanol-infused subjects' PBMC exhibit
ed significantly increased replication of HIV-1 when infected in vitro
, which was associated with increased inhibition of CD8+ T lymphocytes
' function by alcohol.