FORMATION AND CHARACTERIZATION OF A TRANSITION-STATE COMPLEX OF AZOTOBACTER-VINELANDII NITROGENASE

Authors
DUYVIS MG WASSINK H HAAKER H
Citation
Mg. Duyvis et al., FORMATION AND CHARACTERIZATION OF A TRANSITION-STATE COMPLEX OF AZOTOBACTER-VINELANDII NITROGENASE, FEBS letters, 380(3), 1996, pp. 233-236
Citations number
21
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
380
Issue
3
Year of publication
1996
Pages
233 - 236
Database
ISI
SICI code
0014-5793(1996)380:3<233:FACOAT>2.0.ZU;2-Y
Abstract
A stable complex is formed between the nitrogenase proteins of Azotoba cter vinelandii, aluminium fluoride and MgADP. All nitrogenase activit ies are inhibited. The complex formation was found to be reversible. A n incubation at 50 degrees C recovers nitrogenase activity. The comple x has been characterized with respect to protein and nucleotide compos ition and redox state of the metal-sulphur clusters. Based on the inhi bition by aluminium fluoride together with MgADP, it is proposed that a stable transition state complex of nitrogenase is isolated.