SGP28, A NOVEL MATRIX GLYCOPROTEIN IN SPECIFIC GRANULES OF HUMAN NEUTROPHILS WITH SIMILARITY TO A HUMAN TESTIS-SPECIFIC GENE-PRODUCT AND TOA RODENT SPERM-COATING GLYCOPROTEIN

A novel 28 kDa glycoprotein was purified from exocytosed material from human neutrophils and its primary structure partially determined, Deg enerate oligonucleotide primers were used to amplify cDNA clones from a human bone marrow cDNA library, The deduced 245 amino acid sequence of the 2124 bp full-length cDNA showed high degrees of similarity to t he deduced sequences of the human gene TPX-1 and of sperm coating glyc oprotein from rat and mouse, Subcellular fractionation of human neutro phils indicated that the protein is localized in specific granules, Th e protein was named SGP28 (specific granule protein of 28 kDa).