J. Opackajuffry et al., GDNF PROTECTS AGAINST 6-OHDA NIGROSTRIATAL LESION - IN-VIVO STUDY WITH MICRODIALYSIS AND PET, NeuroReport, 7(1), 1995, pp. 348-352
WE have investigated whether glial cell line-derived neurotrophic fact
or (GDNF) protects against a complete unilateral 6-hydroxydopamine (6-
OHDA) nigrostriatal lesion, a robust rat model of Parkinson's disease.
GDNF or vehicle were administered above the rat substantia nigra and
into the lateral ventricle immediately before an ipsilateral 6-OHDA in
jection into the medial forebrain bundle. In vivo tests were employed
to assess the effects of the treatment: microdialysis to measure stria
tal dopamine release, amphetamine challenge to estimate turning behavi
our, and positron emission tomography (PET) to image dopamine reuptake
sites. The present results show that GDNF can protect dopaminergic ne
urones against an acute and irreversible 6-OHDA lesion. They are encou
raging for potential use of GDNF in Parkinson's disease.