DENATURED STATE OF LYSOZYME IN DIMETHYL-S ULFOXIDE

An influence of DMSO on lysozyme structure in solution was studied by fluorescence and optical rotary dispersion methods. Change in the prot ein structure was shown to proceed at DMSO concentration in water grea ter than 60% and result in an increase of the protein helicity. Howeve r,this structural state of lysozyme is similar to that of the unstruct ured peptides obtained by its complete proteolysis and is characterize d by parameters of accessibility to solvent and mobility of their intr insic chromophores. The data obtained evidenced that long range intera ctions have a little influence on the maintenance of the residual seco ndary structure of lysozyme in the presence of DMSO.