SYNTHESIS AND CHARACTERIZATION OF THE 2ND CYSTEINE-RICH REGION OF MOUSE SKIN PKC-ETA

The second cysteine-rich region of mouse skin PKC eta, peptide D, was prepared by automated solid phase peptide synthesis. In the presence o f zinc and phosphatidylserine, peptide D bound [H-3]phorbol 12,13-dibu tyrate with high affinity (K-d = 0.91 nM). Peptide D serves as an effe ctive surrogate for the mouse skin derived receptor, affording unique opportunities for the study of binding, affinity labeling, and solutio n structure related to the PKC regulatory domain.