INCREASE IN LECTIN-BINDING SITES ON EPITHELIAL-CELLS BY CHRONIC BLADDER INFECTION IN RATS

Using lectin histochemistry we assessed whether chronic bladder infect ion modifies carbohydrate residues of glycoconjugates on uroepithelial cells in rats. The bladder infection was produced by implanting a kno tted silk thread with Escherichia coli into the bladder. One or 4 week s after the implantation the bladder was excised, incubated with sixte en biotinylated lectins and stained. The bladder epithelia as a whole stained more strongly positive for eight lectins in the infected rats than in the control rats having a sterile silk thread in the bladder. In the infected rats, the superficial epithelial layer that stained ne gative for Arachis hypogaea (PNA) in the controls became strongly posi tive for PNA, whereas the middle and deep epithelial layers increased in staining for Canavalia ensiformis and six other lectins. These resu lts indicate that chronic bladder infection increases carbohydrate res idues on uroepithelial cells and may facilitate bacterial adherence to uroepithelial cells.