PHARMACOLOGICAL THERAPIES OF INTESTINAL R EPERFUSION INJURY IN ANIMAL-EXPERIMENTS

Summ.: We evaluated experimentally (80 Lewis-rats) possible pharmacolo gical strategies in the treatment of intestinal reperfusion injury in hypo- and normothermia. We used a specific perfusion solution containi ng PGI, or radical scavengers (superoxide dismutase, oxypurinol, tocop herol, ascorbate). Decreased malondialdehyde (MDA) plasma release afte r reperfusion proved the antioxidative efficiency of the administered radical scavengers (normothermia - control group: MDA increase after 1 5 min of reperfusion to 160 +/- 30 % compared to level at the end of i schemia, oxypurinol: 110 +/- 23 %, tocopherol: 112 +/- 12 %, ascorbate : 104 +/- 20 %; p < 0,05), The ATP/ADP-ratio of the therapy groups was stable In contrast to the control group. Alkaline phosphatase release was significantly diminished under radical scavenger administration ( normothermia/l5 min reperfusion - control group: 7,7 +/- 0,9 mu mol/ls , oxypurinol: 4,4 +/- 0,4 mu mol/ls, tocopherol: 3,5 +/- 0,1 mu mol/ls , ascorbate: 5,9 +/- 0,3 mu mol/ls; p < 0,05). Histologically we obser ved a mucosa protective effect particularly in the ascorbate group. Ot her pharmacological strategies are discussed.