N. Oyama et al., GIBBERELLINS AND ANTHERIDIOGENS IN PROTHALLIA AND SPOROPHYTES OF ANEMIA-PHYLLITIDIS, Bioscience, biotechnology, and biochemistry, 60(2), 1996, pp. 301-304
The following gibberellins (GAs) and antheridiogens were identified by
their mass spectra and Kovats retention indices from combined gas chr
omatography-mass spectrometry of purified extracts of the prothallia a
nd sporophytes of Anemia phyllitidis, a Schizaeaceous fern: a trace am
ount of GA(9) (4-week-old prothallia); GA(9), GA(24), GA(25), antherid
ic acid and 3-epi-GA(63) (6-week-old prothallia); and GA(4), GA(9), GA
(15), GA(19), GA(20), and GA(24) [young sporophytes (younger than one
year old) and/or old sporophytes (between one- and two years old), Of
these compounds, GA(24), GA(9), and GA(4) were quantified by gas chrom
atography-selected ion monitoring, using H-2-GAs as internal standards
, and the content of antheridic acid, the principal antheridiogen, was
evaluated by a radioimmunoassay which we have developed, The results
indicate that endogenous levels of GAs and antheridiogens in prothalli
a began to increase rapidly between 4 and 6 weeks after sowing, the co
ntents of antheridic acid and GA(24), the most abundant GA in 6-week-o
ld prothallia, being 107.4 and 37.9 ng/g fresh weight, respectively, T
he most abundant GA in the sporophytes was GA(9), the content in young
and old sporophytes being 15.3 and 7.3 ng/g fresh weight, respectivel
y.