CLONING AND SEQUENCING OF A RICE GENE ENCODING THE 13-KDA PROLAMIN POLYPEPTIDE

A gene encoding the 13-kDa prolamin polypeptide was isolated from a ri ce genomic library (lambda EMBL3) and the nucleotide sequence of an ab out 3-kbp EcoRI fragment was analyzed. The cloned gene (NRP33) codes f or a protein composed of 156 amino acids, including a signal peptide o f 19 amino acid residues and no intron is present in the genomic clone , The nucleotide sequence contains consensus TATA and CAAT boxes, and two polyadenylation signals, In addition, there are two conserved sequ ences named the -300 element and 10 consecutive repeats of the trinucl eotide ATT in the 5' noncoding sequence.