IDENTIFICATION OF THE GLYCOSYLATION SITE OF A MAJOR SOYBEAN ALLERGEN,GLY-M BD 30K

Authors
BANDO N TSUJI H YAMANISHI R NIO N OGAWA T
Citation
N. Bando et al., IDENTIFICATION OF THE GLYCOSYLATION SITE OF A MAJOR SOYBEAN ALLERGEN,GLY-M BD 30K, Bioscience, biotechnology, and biochemistry, 60(2), 1996, pp. 347-348
Citations number
7
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
2
Year of publication
1996
Pages
347 - 348
Database
ISI
SICI code
0916-8451(1996)60:2<347:IOTGSO>2.0.ZU;2-I
Abstract
A major soybean allergen, Gly In Ed 30K, is a glycoprotein, A peptide containing a sugar chain was isolated from an alpha-chymotrypic hydrol yzate of the allergen. The amino acid sequence analysis of the peptide showed that its sugar chain binds to the Asn(170) residue. Furthermor e, the sugar moiety of the allergen and peptide,vas shown to consist o f mannose, N-acetylglucosamine, fucose, and xylose at a molar ratio of 3:2:1:1, These results indicate that Gly m Bd 30K is a N-linked glyco protein.