SEQUENCE OF THE BACILLUS-STEAROTHERMOPHILUS GENE ENCODING ASPARTOKINASE-II

Authors
CANTONI R LABO M DEROSSI E RICCARDI G
Citation
R. Cantoni et al., SEQUENCE OF THE BACILLUS-STEAROTHERMOPHILUS GENE ENCODING ASPARTOKINASE-II, Gene, 169(1), 1996, pp. 135-136
Citations number
5
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
169
Issue
1
Year of publication
1996
Pages
135 - 136
Database
ISI
SICI code
0378-1119(1996)169:1<135:SOTBGE>2.0.ZU;2-R
Abstract
The complete nucleotide sequence of the gene encoding aspartokinase (A sk) II from thermophilic Bacillus stearothermophilus has been determin ed. Degenerate oligodeoxyribonucleotides primed the amplification of a 932-bp gene. This sequence was successively used for constructing new primers applied in inverse polymerase chain reaction using, as templa te, self-ligating DNA fragments. The deduced amino-acid sequence is 68 .7% identical with the sequence of the Bacillus sp. strain MGA.3 Ask I I.