A FUNCTIONAL SPO0A IS REQUIRED FOR MAXIMAL APRE EXPRESSION IN BACILLUS-SUBTILIS

The initiation of sporulation in Bacillus subtilis is under control of the transcriptional factor Spo0A. Most Spo0A mutants fail to initiate the sporulation process and all the sporulation initiated processes s uch as the synthesis of subtilisin, However, the product of spo0A9V, o ne of the several spo0A mutants characterized, distinguishes itself in the fact that, while it appears to effectively repress abrB, it fails to activate the spoIIA operon, The aim of this study was to examine t he effect of the spo0A9V mutation on aprE expression and we found that in different genetic backgrounds, the spo0A9V mutation has a negative effect on aprE::lacZ expression.