NF-KAPPA-B P50 SUBUNIT CROSS-LINKING TO DNA DUPLEXES, CONTAINING A MONOSUBSTITUTED PYROPHOSPHATE INTERNUCLEOTIDE BOND

The new express technique based on the use of BrCN to synthesize DNA d uplexes, containing non-substituted or monosubstituted pyrophosphate i nternucleotide bonds has been proposed, Using this technique, DNA dupl exes having modified internucleotide bonds between dT and dC residues in the human NF-kappa B transcription factor recognition sequence in H IV-1 (5'-GGAAAGTCCC-3') have been prepared. We demonstrate that these internucleotide bonds within the recognition site do not prevent the f ormation of NF-kappa B p50 subunit complex with the corresponding dupl exes. The cross-linking of NF-kappa B p50 subunit to the DIVA duplex c ontaining a monosubstituted pyrophosphate internucleotide bond has bee n successfully performed.