MAPPING OF DNA-BINDING PROTEINS ALONG THE YEAST GENOME BY UV-INDUCED DNA-PROTEIN CROSS-LINKING

UV-induced crosslinking of DNA-binding proteins to DNA in intact nucle i of Saccharomyces cerevisiae and subsequent 'protein image' hybridiza tion were applied to map nonhistone proteins along single-copy genes o f yeast, We detected two polypeptides that most probably correspond to core subunits of yeast RNA-polymerase II in the coding region of tran sketolase gene (TKL2), Several non-histone proteins were also detected which bind to the upstream region of TKL2 gene, and to the intergenic spacer between calmodulin (CMD1) and beta-mannosyl transferase (ALG1) genes.