Electron tomography is a powerful tool in elucidating the three-dimens
ional architecture of large biological complexes and subcellular organ
elles. Its use can be expanded through the simplification of the tomog
raphic procedure by automation of its tasks. In this paper, we describ
e our EMACT/EMCAT system, which automates both tomographic data collec
tion and reconstruction. (C) 1996 Academic Press, Inc.