The three-dimensional reconstruction of icosahedral viruses from elect
ron micrograph images has become an important tool for understanding v
irus structure, function, and pathogenesis. We have developed an integ
rated suite of software programs to automate most of the operations in
volved in producing these reconstructions from EM images. Our package
combines the analytical capabilities of preexisting algorithms togethe
r with approaches we have developed to produce an interactive working
environment which enhances the efficiency and usefulness of this appro
ach to the structural analysis of icosahedral viruses. (C) 1996 Academ
ic Press, Inc.