INHIBITION OF GLYCOLYSIS IN BOAR SPERMATOZOA BY ALPHA-CHLOROHYDRIN PHOSPHATE APPEARS TO BE MEDIATED BY PHOSPHATASE-ACTIVITY

Authors
JONES AR PORTER LM
Citation
Ar. Jones et Lm. Porter, INHIBITION OF GLYCOLYSIS IN BOAR SPERMATOZOA BY ALPHA-CHLOROHYDRIN PHOSPHATE APPEARS TO BE MEDIATED BY PHOSPHATASE-ACTIVITY, Reproduction, fertility and development, 7(5), 1995, pp. 1089-1094
Citations number
29
Categorie Soggetti
Reproductive Biology","Developmental Biology
Journal title
Reproduction, fertility and development → ACNP
ISSN journal
10313613
Volume
7
Issue
5
Year of publication
1995
Pages
1089 - 1094
Database
ISI
SICI code
1031-3613(1995)7:5<1089:IOGIBS>2.0.ZU;2-O
Abstract
(R,S)-alpha-chlorohydrin-1-phosphate, previously shown to have no anti -glycolytic activity on mature boar sperm in vitro, is a substrate for acid and/or neutral phosphatase(s) that are associated with washed sp erm. The high phosphatase activity hydrolyses the ester to alpha-chlor ohydrin which undergoes oxidation to (S)-3-chlorolactaldehyde, a speci fic inhibitor of sperm glyceraldehyde-3-phosphate dehydrogenase and tr iosephosphate isomerase, thereby exhibiting an anti-glycolytic action.