COMPLEX ARRANGEMENT OF DISPERSED REPEATED DNA-SEQUENCES IN ORYZA-OFFICINALIS

A 525-bp BglII fragment was isolated from Oryza officinalis DNA (acces sion W1278) and shown to correspond to a new dispersed repetitive DNA sequence with specificity restricted to a subset of the wild rice with a C genome. The sequence of the fragment was determined but it does n ot correspond to any sequence already present in databases. It contain s several imperfect palindromes. Larger genomic clones (12-18 kbp) wer e isolated and all contain sequences homologous to the BglII element. Analysis of these clones confirms that the BglII element is dispersed in the O. officinalis genome. From one genomic clone, the sequences ad jacent to the BglII element were subcloned and used as probes to demon strate that the sequences flanking the BglII element are variable in d ifferent genomic clones and that some of them are also dispersed repet itive sequences. The genomic specificity of two of these dispersed rep eats was evaluated and shown to be different from that of the initial BglII element. This analysis revealed a complex arrangement of various dispersed repeated sequences.