CYTOTOXICITY OF B72.3XOKT3 BISPECIFIC ANTIBODY RECOGNIZING HUMAN COLON-CANCER

Bispecific monoclonal antibodies can be used to redirect peripheral bl ood lymphocytes against tumor cells. In the present study, a murine bi specific monoclonal antibody was developed using somatic hybrydization . The antibody has two different binding arms: one arm directed agains t human CD3 receptor expressed on T-lymphocytes and the other against tumor associated glycoprotein TAG-72, expressed on human carcinomas, s uch as colon, breast, and pancreas. Partially purified antibody was ca pable of inducing human T-cell proliferation and preventing growth of colon cancer cell line in nu/nu mice in a tumor neutralization assay. (C) 1996 Academic Press, Inc.