DETECTION OF PROLIFERATING CELL NUCLEAR ANTIGEN IN GLIOMAS AND ADJACENT RESECTION MARGINS

Citation
Dk. Kim et al., DETECTION OF PROLIFERATING CELL NUCLEAR ANTIGEN IN GLIOMAS AND ADJACENT RESECTION MARGINS, Neurosurgery, 33(4), 1993, pp. 619-626
Citations number
49
Categorie Soggetti
Surgery,Neurosciences
Journal title
ISSN journal
0148396X
Volume
33
Issue
4
Year of publication
1993
Pages
619 - 626
Database
ISI
SICI code
0148-396X(1993)33:4<619:DOPCNA>2.0.ZU;2-J
Abstract
WE DESCRIBE A technique for estimating the number of proliferating cel ls in gliomas and adjacent resection cavities after tumor removal. Pro liferating cell nuclear antigen (PCNA) is a nuclear protein associated with the cell cycle. Anti-PCNA antibody staining provides a semiquant itative estimate of the number of proliferating cells found in fixed t issue embedded in paraffin. The extent of the staining of tumor cells of glioblastoma multiforme, anaplastic astrocytomas, low-grade gliomas , and other lesions of the brain with anti-PCNA antibody is correlated with the histological diagnosis. In addition, the labeling of the mar gins after resection of gliomas and other lesions with anti-PCNA antib ody is also associated with the histological diagnosis of the lesion. This technique may be useful in estimating the ''biological'' extent o f resection and in predicting the recurrence patterns of gliomas.