PYRIDOXAL-PHOSPHATE INDUCED ASSOCIATION REACTIONS OF ADRENODOXIN AND ADRENODOXIN-REDUCTASE

Pyridoxal phosphate, cofactor of several enzymes, possesses linking pr operties to induce oligomerization of identical proteins such as adren odoxin-reductase or adrenodoxin. The capability to get such self-assem blies is slightly lower than that for obtaining the heterologous compl ex between adrenodoxin-reductase and adrenodoxin which was found to he essential for electron transfer in the cytochrome P450 system. The in fluence of pyridoxal phosphate on the complex formation between adreno doxin-reductase and adrenodoxin as well as the oligomerization reactio n of the isolated proteins and possible consequences is discussed. (C) 1996 Academic Press, Inc.